Impact of three commercial feed formulations on farmed gilthead sea bream (Sparus aurata, L.) metabolism as inferred from liver and blood serum proteomics

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dc.contributor.authorGhisaura, Stefania
dc.contributor.authorAnedda, Roberto
dc.contributor.authorPagnozzi, Daniela
dc.contributor.authorBiosa, Grazia
dc.contributor.authorSpada, Simona
dc.contributor.authorBonaglini, Elia
dc.contributor.authorCappuccinelli, Roberto
dc.contributor.authorRoggio, Tonina
dc.contributor.authorUzzau, Sergio
dc.contributor.authorAddis, Maria Filippa
dc.date.accessioned2014-12-09T13:09:39Z
dc.date.available2014-12-09T13:09:39Z
dc.date.issued2014-09-24
dc.description.abstractBackground: The zootechnical performance of three different commercial feeds and their impact on liver and serum proteins of gilthead sea bream (Sparus aurata, L.) were assessed in a 12 week feeding trial. The three feeds, named A, B, and C, were subjected to lipid and protein characterization by gas chromatography (GC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. Results: Feed B was higher in fish-derived lipids and proteins, while feeds C and A were higher in vegetable components, although the largest proportion of feed C proteins was represented by pig hemoglobin. According to biometric measurements, the feeds had significantly different impacts on fish growth, producing a higher average weight gain and a lower liver somatic index in feed B over feeds A and C, respectively. 2D DIGE/MS analysis of liver tissue and Ingenuity pathways analysis (IPA) highlighted differential changes in proteins involved in key metabolic pathways of liver, spanning carbohydrate, lipid, protein, and oxidative metabolism. In addition, serum proteomics revealed interesting changes in apolipoproteins, transferrin, warm temperature acclimation-related 65 kDa protein (Wap65), fibrinogen, F-type lectin, and alpha-1-antitrypsin. Conclusions: This study highlights the contribution of proteomics for understanding and improving the metabolic compatibility of feeds for marine aquaculture, and opens new perspectives for its monitoring with serological tests.IT
dc.description.statusPubblicatoIT
dc.identifier.doi10.1186/s12953-014-0044-3IT
dc.identifier.eissn1477-5956
dc.identifier.issn1477-5956
dc.identifier.urihttp://hdl.handle.net/11050/1134
dc.language.isoenIT
dc.publisherBioMed CentralIT
dc.relation.ispartofProteome ScienceIT
dc.relation.ispartofseries12;44
dc.rightsAttribuzione - Non commerciale - Non opere derivate 3.0 Italia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/it/*
dc.subjectgilthead sea breamIT
dc.subjectaquacultureIT
dc.subjectfish feedIT
dc.subjectfarmed fishIT
dc.subjectliver proteinsIT
dc.subjectserum proteinsIT
dc.subject2D DIGEIT
dc.subjectmass spectrometryIT
dc.subjectingenuity pathway analysisIT
dc.subjectproteomicsIT
dc.subject.een-cordisEEN CORDIS::SCIENZE BIOLOGICHE ::Ricerca sul genoma ::Espressione genica, ricerca proteomicaIT
dc.subject.een-cordisEEN CORDIS::AGRICOLTURA E RISORSE MARINE::Risorse del mare, della pesca ::AcquacolturaIT
dc.titleImpact of three commercial feed formulations on farmed gilthead sea bream (Sparus aurata, L.) metabolism as inferred from liver and blood serum proteomicsIT
dc.typeArticoloIT
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