Molecular changes induced by the curcumin analogue D6 in human melanoma cells

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dc.contributor.authorRozzo, Carla
dc.contributor.authorFanciulli, Manuela
dc.contributor.authorFraumene, Cristina
dc.contributor.authorCorrias, Antonio
dc.contributor.authorCubeddu, Tiziana
dc.contributor.authorSassu, Ilaria
dc.contributor.authorCossu, Sara
dc.contributor.authorNieddu, Valentina
dc.contributor.authorGalleri, Grazia
dc.contributor.authorAzara, Emanuela
dc.contributor.authorDettori, Maria Antonietta
dc.contributor.authorFabbri, Davide
dc.contributor.authorPalmieri, Giuseppe
dc.contributor.authorPisano, Marina
dc.date.accessioned2015-01-22T13:10:20Z
dc.date.available2015-01-22T13:10:20Z
dc.date.issued2013-05-04
dc.description.abstractBackground: In a previous report, we described the in vitro and in vivo antiproliferative and proapoptotic activity of a hydroxylated biphenyl (D6), a structural analogue of curcumin, on malignant melanoma and neuroblastoma tumours. In this paper, we investigated the molecular changes induced by such a compound, underlying cell growth arrest and apoptosis in melanoma cells. Results: To shed light on the mechanisms of action of D6, we firstly demonstrated its quick cellular uptake and subsequent block of cell cycle in G2/M phase transition. A gene expression profile analysis of D6-treated melanoma cells and fibroblasts was then carried out on high density microarrays, to assess gene expression changes induced by this compound. The expression profile study evidenced both an induction of stress response pathways and a modulation of cell growth regulation mechanisms. In particular, our data suggest that the antiproliferative and proapoptotic activities of D6 in melanoma could be partially driven by up-regulation of the p53 signalling pathways as well as by down-regulation of the PI3K/Akt and NF-kB pathways. Modulation of gene expression due to D6 treatment was verified by western blot analysis for single proteins of interest, confirming the results from the gene expression profile analysis. Conclusions: Our findings contribute to the understanding of the mechanisms of action of D6, through a comprehensive description of the molecular changes induced by this compound at the gene expression level, in agreement with the previously reported anti-tumour effects on melanoma cells.IT
dc.description.statusPubblicatoIT
dc.identifier.doi10.1186/1476-4598-12-37IT
dc.identifier.issn1476-4598
dc.identifier.urihttp://hdl.handle.net/11050/1144
dc.language.isoenIT
dc.publisherBioMed CentralIT
dc.relation.ispartofMolecula CancerIT
dc.relation.ispartofseries12;37
dc.rightsAttribuzione - Non commerciale - Non opere derivate 3.0 Italia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/it/*
dc.subjectdrug effectsIT
dc.subjectmelanomaIT
dc.subjectcurcuminIT
dc.subjecthydroxylated biphenylIT
dc.subjectD6IT
dc.subjectantineoplastic agentsIT
dc.subject.een-cordisEEN CORDIS::SCIENZE BIOLOGICHE ::Ricerca sul genoma ::Espressione genica, ricerca proteomicaIT
dc.titleMolecular changes induced by the curcumin analogue D6 in human melanoma cellsIT
dc.typeArticoloIT
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