The MEPS server for identifying protein conformational epitopes
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Data
2007-03-08
Autori
Castrignanò, Tiziana
D'Onorio de Meo, Paolo
Carrabino, Danilo
Orsini, Massimiliano
Floris, Matteo
Tramontano, Anna
Titolo del periodico
ISSN
Titolo del volume
Editore
BioMed Central
Abstract
One of the most interesting problems in molecular immunology is epitope mapping, i.e. the
identification of the regions of interaction between
an antigen and an antibody. The solution to this problem, even if approximate, would help in designing experiments to precisely map the residues involved in the interaction and could be instrumental both in designing peptides able to
mimic the interacting surface of the antigen and in
understanding where immunologically important regions are located in its three-dimensional structure. From an experimental point of view, both genetically encoded and chemically synthesised peptide libraries can be used to identify sequences recognized by a given antibody. The problem then arises of which region of a folded protein the
selected peptides correspond to. RESULTS: We have developed a method able to find the surface region of a protein that can be effectively mimicked by a peptide, given the structure of the protein and the maximum number of side chains deemed to be required for recognition. The method is implemented as a publicly available server. It can also find and report all peptide sequences of a specified length
that can mimic the surface of a given protein and store them in a database.The immediate application of the server is the mapping of antibody epitopes, however the system is sufficiently flexible for allowing other questions to be asked, for example one can compare the peptides representing the surface of two proteins known to interact with the same macromolecule to find which is the most likely
interacting region. CONCLUSION: We believe that the MEPS server, available at http://www.caspur.it/meps, will be a useful tool for immunologists and structural and computational biologists. We plan to use it ourselves to implement a database of "surface mimicking peptides" for all proteins of known structure and proteins that can be reliably modelled by comparative modelling.
Descrizione
Keywords
antibodies chemistry immunology , computational biology methods , epitope mapping methods , protein conformation , proteins chemistry immunology , sequence alignment methods , sequence analysis protein methods