An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid

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dc.contributor.authorPagnozzi, Daniela
dc.contributor.authorBiosa, Grazia
dc.contributor.authorAddis, Maria Filippa
dc.contributor.authorMastrandrea, Scilla
dc.contributor.authorMasala, Giovanna
dc.contributor.authorUzzau, Sergio
dc.date.accessioned2014-12-09T12:00:44Z
dc.date.available2014-12-09T12:00:44Z
dc.date.issued2014-08-13
dc.description.abstractBackground: Currently, the serodiagnosis of cystic echinococcosis relies mostly on crude Echinococcus granulosus hydatid cyst fluid as the antigen. Consequently, available immunodiagnostic tests lack standardization of the target antigen and, in turn, this is reflected on poor sensitivity and specificity of the serological diagnosis. Methodology/Principal Findings: Here, a chromatographic method enabling the generation of highly enriched Antigen 5 (Ag5) is described. The procedure is very easy, efficient and reproducible, since different hydatid cyst fluid (HCF) sources produced very similar chromatograms, notwithstanding the clearly evident and extreme heterogeneity of the starting material. In addition, the performance of the antigen preparation in immunological assays was preliminarily assessed by western immunoblotting and ELISA on a limited panel of cystic echinococcosis patients and healthy controls. Following western immunoblotting and ELISA experiments, a high reactivity of patient sera was seen, with unambiguous and highly specific results. Conclusions/Significance: The methods and results reported open interesting perspectives for the development of sensitive diagnostic tools to enable the timely and unambiguous detection of cystic echinococcosis antibodies in patient sera.IT
dc.description.sponsorshipThis work was supported by Regione Autonoma della Sardegna (http://www.regione.sardegna.it/)IT
dc.description.statusPubblicatoIT
dc.identifier.citationPagnozzi D, Biosa G, Addis MF, Mastrandrea S, Masala G, et al. (2014) An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid. PLoS ONE 9(8): e104962. doi:10.1371/journal.pone.0104962IT
dc.identifier.doi10.1371/journal.pone.0104962IT
dc.identifier.eissn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11050/1132
dc.language.isoenIT
dc.publisherPublic Library of ScienceIT
dc.relation.ispartofPLoS ONEIT
dc.relation.ispartofseries9;8
dc.rightsAttribuzione - Non commerciale - Non opere derivate 3.0 Italia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/it/*
dc.subjectantigensIT
dc.subjectdiagnostic medicineIT
dc.subjectechinococcosisIT
dc.subjectenzyme-linked immunoassaysIT
dc.subjectserum proteinsIT
dc.subjectmembrane proteinsIT
dc.subjectsheepIT
dc.subjectsize-exclusion chromatographyIT
dc.subject.een-cordisEEN CORDIS::SCIENZE BIOLOGICHE ::Medicina, salute umana::Apparecchiature medicali ed elettromedicaliIT
dc.subject.een-cordisEEN CORDIS::SCIENZE BIOLOGICHE ::Ricerca sul genoma ::Espressione genica, ricerca proteomicaIT
dc.titleAn Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst FluidIT
dc.typeArticoloIT
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