Influence of Moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (Sparus aurata, L.)
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Data
2010-10-12
Autori
Addis, Maria Filippa
Cappuccinelli, Roberto
Tedde, Vittorio
Pagnozzi, Daniela
Viale, Iolanda
Meloni, Mauro
Salati, Fulvio
Roggio, Tonina
Uzzau, Sergio
Titolo del periodico
ISSN
Titolo del volume
Editore
BioMed Central
Abstract
Background: Currently, presence of Moraxella sp. in internal organs of fish is not considered detrimental for fish
farming. However, bacterial colonization of internal organs can affect fish wellness and decrease growth rate, stress
resistance, and immune response. Recently, there have been reports by farmers concerning slow growth, poor feed
conversion, and low average weight increase of fish farmed in offshore floating sea cages, often associated with
internal organ colonization by Moraxella sp. Therefore, presence of these opportunistic bacteria deserves further
investigations for elucidating incidence and impact on fish metabolism.
Results: A total of 960 gilthead sea breams (Sparus aurata, L.), collected along 17 months from four offshore sea
cage plants and two natural lagoons in Sardinia, were subjected to routine microbiological examination of internal
organs throughout the production cycle. Thirteen subjects (1.35%) were found positive for Moraxella sp. in the
kidney (7), brain (3), eye (1), spleen (1), and perivisceral fat (1). In order to investigate the influence of Moraxella sp.
colonization, positive and negative kidney samples were subjected to a differential proteomics study by means of
2-D PAGE and mass spectrometry. Interestingly, Moraxella sp. infected kidneys displayed a concerted upregulation
of several mitochondrial enzymes compared to negative tissues, reinforcing previous observations following
lipopolysaccharide (LPS) challenge in fish.
Conclusions: Presence of Moraxella sp. in farmed sea bream kidney is able to induce proteome alterations similar
to those described following LPS challenge in other fish species. This study revealed that Moraxella sp. might be
causing metabolic alterations in fish, and provided indications on proteins that could be investigated as markers of
infection by Gram-negative bacteria within farming plants.
Descrizione
Keywords
Moraxella sp. , sparus aurata , Sardinia , alterations in fish