Influence of Moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (Sparus aurata, L.)

Visualizzazione ridotta
dc.contributor.authorAddis, Maria Filippa
dc.contributor.authorCappuccinelli, Roberto
dc.contributor.authorTedde, Vittorio
dc.contributor.authorPagnozzi, Daniela
dc.contributor.authorViale, Iolanda
dc.contributor.authorMeloni, Mauro
dc.contributor.authorSalati, Fulvio
dc.contributor.authorRoggio, Tonina
dc.contributor.authorUzzau, Sergio
dc.date.accessioned2014-12-16T11:05:49Z
dc.date.available2014-12-16T11:05:49Z
dc.date.issued2010-10-12
dc.description.abstractBackground: Currently, presence of Moraxella sp. in internal organs of fish is not considered detrimental for fish farming. However, bacterial colonization of internal organs can affect fish wellness and decrease growth rate, stress resistance, and immune response. Recently, there have been reports by farmers concerning slow growth, poor feed conversion, and low average weight increase of fish farmed in offshore floating sea cages, often associated with internal organ colonization by Moraxella sp. Therefore, presence of these opportunistic bacteria deserves further investigations for elucidating incidence and impact on fish metabolism. Results: A total of 960 gilthead sea breams (Sparus aurata, L.), collected along 17 months from four offshore sea cage plants and two natural lagoons in Sardinia, were subjected to routine microbiological examination of internal organs throughout the production cycle. Thirteen subjects (1.35%) were found positive for Moraxella sp. in the kidney (7), brain (3), eye (1), spleen (1), and perivisceral fat (1). In order to investigate the influence of Moraxella sp. colonization, positive and negative kidney samples were subjected to a differential proteomics study by means of 2-D PAGE and mass spectrometry. Interestingly, Moraxella sp. infected kidneys displayed a concerted upregulation of several mitochondrial enzymes compared to negative tissues, reinforcing previous observations following lipopolysaccharide (LPS) challenge in fish. Conclusions: Presence of Moraxella sp. in farmed sea bream kidney is able to induce proteome alterations similar to those described following LPS challenge in other fish species. This study revealed that Moraxella sp. might be causing metabolic alterations in fish, and provided indications on proteins that could be investigated as markers of infection by Gram-negative bacteria within farming plants.IT
dc.description.statusPubblicatoIT
dc.identifier.doi10.1186/1477-5956-8-50IT
dc.identifier.issn1477-5956
dc.identifier.urihttp://hdl.handle.net/11050/1135
dc.language.isoenIT
dc.publisherBioMed CentralIT
dc.relation.ispartofProteome scienceIT
dc.relation.ispartofseries8;50
dc.rightsAttribuzione - Non commerciale - Non opere derivate 3.0 Italia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/it/*
dc.subjectMoraxella sp.IT
dc.subjectsparus aurataIT
dc.subjectSardiniaIT
dc.subjectalterations in fishIT
dc.subject.een-cordisEEN CORDIS::SCIENZE BIOLOGICHE ::Biologia / biotecnologia ::MicrobiologiaIT
dc.subject.een-cordisEEN CORDIS::AGRICOLTURA E RISORSE MARINE::Risorse del mare, della pesca ::AcquacolturaIT
dc.titleInfluence of Moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (Sparus aurata, L.)IT
dc.typeArticoloIT
File
Original bundle
Ora in mostra 1 - 1 di 1
Immagine di anteprima
Nome:
1477-5956-8-50.pdf
Dimensione:
721.25 KB
Formato:
Adobe Portable Document Format
Descrizione:
Download
License bundle
Ora in mostra 1 - 1 di 1
Immagine di anteprima
Nome:
license.txt
Dimensione:
2.06 KB
Formato:
Item-specific license agreed upon to submission
Descrizione:
Download
collections
PCR Articolo