Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection

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dc.contributor.authorCacciotto, Carla
dc.contributor.authorAddis, Maria Filippa
dc.contributor.authorCoradduzza, Elisabetta
dc.contributor.authorCarcangiu, Laura
dc.contributor.authorNuvoli, Anna Maria
dc.contributor.authorTore, Gessica
dc.contributor.authorDore, Gian Mario
dc.contributor.authorPagnozzi, Daniela
dc.contributor.authorUzzau, Sergio
dc.contributor.authorChessa, Bernardo
dc.contributor.authorPittau, Marco
dc.contributor.authorAlberti, Alberto
dc.date.accessioned2014-12-17T10:35:07Z
dc.date.available2014-12-17T10:35:07Z
dc.date.issued2013-02-28
dc.description.abstractIn this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed in Escherichia coli as a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2 at temperatures ranging from 37 to 45°C. According to in silico analyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to the Mycoplasma hominis group. In M. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role in Mycoplasma agalactiae survival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants.IT
dc.description.statusPubblicatoIT
dc.identifier.citationCacciotto C, Addis MF, Coradduzza E, Carcangiu L, Nuvoli AM, et al. (2013) Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection. PLoS ONE 8(2): e57775IT
dc.identifier.doi10.1371/journal.pone.0057775IT
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11050/1141
dc.language.isoenIT
dc.publisherPublic Library of ScienceIT
dc.relation.ispartofPLoS ONEIT
dc.relation.ispartofseries8;2
dc.rightsAttribuzione - Non commerciale - Non opere derivate 3.0 Italia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/it/*
dc.subjectMycoplasma agalactiaeIT
dc.subjectsheepIT
dc.subjectrecombinant proteinsIT
dc.subjectMAG_5040IT
dc.subjectEscherichia coliIT
dc.subjectnucleic acidsIT
dc.subject.een-cordisEEN CORDIS::SCIENZE BIOLOGICHE ::Biologia / biotecnologia ::MicrobiologiaIT
dc.subject.een-cordisEEN CORDIS::AGRICOLTURA E RISORSE MARINE::Agricoltura::Produzione animale / allevamentoIT
dc.titleMycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural InfectionIT
dc.typeArticoloIT
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