Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection
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Data
2013-02-28
Autori
Cacciotto, Carla
Addis, Maria Filippa
Coradduzza, Elisabetta
Carcangiu, Laura
Nuvoli, Anna Maria
Tore, Gessica
Dore, Gian Mario
Pagnozzi, Daniela
Uzzau, Sergio
Chessa, Bernardo
Titolo del periodico
ISSN
Titolo del volume
Editore
Public Library of Science
Abstract
In this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed in Escherichia coli as a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2 at temperatures ranging from 37 to 45°C. According to in silico analyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to the Mycoplasma hominis group. In M. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role in Mycoplasma agalactiae survival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants.
Descrizione
Keywords
Mycoplasma agalactiae , sheep , recombinant proteins , MAG_5040 , Escherichia coli , nucleic acids
Citazione
Cacciotto C, Addis MF, Coradduzza E, Carcangiu L, Nuvoli AM, et al. (2013) Mycoplasma agalactiae MAG_5040 is a Mg2+ -Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection. PLoS ONE 8(2): e57775