Porto Conte Ricerche

URI permanente di questa Community

https://hdl.handle.net/11050/3
Porto Conte Ricerche è uno dei motori del Parco Scientifico e Tecnologico della Sardegna, attivo in ambiti tecnologici tra loro complementari: biomarker discovery, sistemi diagnostici e biotecnologie applicate per l’alimentazione e per la salute.

Il Centro di Ricerca sviluppa ed eroga servizi ad alto contenuto tecnologico a favore di imprese impegnate nell’innovazione dei propri sistemi produttivi attraverso attività di ricerca industriale e sviluppo sperimentale.

Sito istituzionale di Porto Conte Ricerche

Sfogliare

Mostra il contenuto di Porto Conte Ricerche per Autore "Azara, Emanuela"

Ora in mostra 1 - 1 di 1
  • Immagine di anteprima
    Item
    Molecular changes induced by the curcumin analogue D6 in human melanoma cells
    (BioMed Central, 2013-05-04) Rozzo, Carla; Fanciulli, Manuela; Fraumene, Cristina; Corrias, Antonio; Cubeddu, Tiziana; Sassu, Ilaria; Cossu, Sara; Nieddu, Valentina; Galleri, Grazia; Azara, Emanuela; Dettori, Maria Antonietta; Fabbri, Davide; Palmieri, Giuseppe; Pisano, Marina
    Background: In a previous report, we described the in vitro and in vivo antiproliferative and proapoptotic activity of a hydroxylated biphenyl (D6), a structural analogue of curcumin, on malignant melanoma and neuroblastoma tumours. In this paper, we investigated the molecular changes induced by such a compound, underlying cell growth arrest and apoptosis in melanoma cells. Results: To shed light on the mechanisms of action of D6, we firstly demonstrated its quick cellular uptake and subsequent block of cell cycle in G2/M phase transition. A gene expression profile analysis of D6-treated melanoma cells and fibroblasts was then carried out on high density microarrays, to assess gene expression changes induced by this compound. The expression profile study evidenced both an induction of stress response pathways and a modulation of cell growth regulation mechanisms. In particular, our data suggest that the antiproliferative and proapoptotic activities of D6 in melanoma could be partially driven by up-regulation of the p53 signalling pathways as well as by down-regulation of the PI3K/Akt and NF-kB pathways. Modulation of gene expression due to D6 treatment was verified by western blot analysis for single proteins of interest, confirming the results from the gene expression profile analysis. Conclusions: Our findings contribute to the understanding of the mechanisms of action of D6, through a comprehensive description of the molecular changes induced by this compound at the gene expression level, in agreement with the previously reported anti-tumour effects on melanoma cells.