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- ItemProgetto INNOQUINOA_articolo: Effect of Substitution of Rice Flour with Quinoa Flour on the Chemical‐Physical, Nutritional, Volatile and Sensory Parameters of Gluten‐Free Ladyfinger Biscuits(2020-06-19) Fadda, Costantino; Cannas, Michela; Pulina, Simone; Conte, Paola; Del Caro, Alessandra; Urgeghe, Pietro Paolo; Piga, Antonio; Dipartimento di Agraria, Università degli Studi di Sassari
- ItemProgetto IBERNAT-NBL_ articolo: Valproic acid upregulates the expression of the p75NTR/sortilin receptor complex to induce neuronal apoptosis(2020-10-25) ONALI, PIERLUIGI; UNIVERSITA' DEGLI STUDI DI CAGLIARIThe antiepileptic and mood stabilizer agent valproic acid (VPA) has been shown to exert anti-tumour effects and to cause neuronal damage in the developing brain through mechanisms not completely understood. In the present study we show that prolonged exposure of SH-SY5Y and LAN-1 human neuroblastoma cells to clinically relevant concentrations of VPA caused a marked induction of the protein and transcript levels of the common neurotrophin receptor p75NTR and its co-receptor sortilin, two promoters of apoptotic cell death in response to proneurotrophins. VPA induction of p75NTR and sortilin was associated with an increase in plasma membrane expression of the receptor proteins and was mimicked by cell treatment with several histone deacetylase (HDAC) inhibitors. VPA and HDAC1 knockdown decreased the level of EZH2, a core component of the polycomb repressive complex 2, and upregulated the transcription factor CASZ1, a positive regulator of p75NTR. CASZ1 knockdown attenuated VPA-induced p75NTR overexpression. Cell treatment with VPA favoured proNGF-induced p75NTR/sortilin interaction and the exposure to proNGF enhanced JNK activation and apoptotic cell death elicited by VPA. Depletion of p75NTR or addition of the sortilin agonist neurotensin to block proNGF/sortilin interaction reduced the apoptotic response to VPA and proNGF. Exposure of mouse cerebellar granule cells to VPA upregulated p75NTR and sortilin and induced apoptosis which was enhanced by proNGF. These results indicate that VPA upregulates p75NTR apoptotic cell signalling through an epigenetic mechanism involving HDAC inhibition and suggest that this effect may contribute to the anti-neuroblastoma and neurotoxic effects of VPA.
- ItemProgetto Nur_Way: WP2_HANDBOOK(Archeo - Timeline Publishing, 2021-04-27) Depalmas, Anna; Fadda, Noemi; Fundoni, Giovanna; Casu, Filippo; Delogu, Carmen; De Martini, Maria Giovanna; Doro, Luca; Minchilli, Maurizio; Spano, Miriam; Tedeschi, Loredana F.; Università degli Studi di Sassari; Cooperativa Ortuabis - Meana Sardo; Cooperativa Siendas - Thiesi; Società Balares - Tempio PausaniaIl contributo costituisce una narrazione sulla preistoria e protostoria della Sardegna con un particolare focus sui nuraghi. Il testo si configura come un handbook, ossia una sorta di agile guida sui nuraghi e sui monumenti protostorici della Sardegna. Nella trattazione sono inserite le schede dei monumenti gestiti dalle aziende partner del progetto cluster nonché le informazioni su tutte le imprese aderenti al cluster.
- ItemImproving the quality of dough obtained with old durum wheat using hydrocolloids - Pubblicazione scientifica prodotta nell’ambito delle attività di disseminazione (WP 6) del progetto cluster "AVIPROFO"(Elsevier, 2019-10-24) Farbo, Maria Grazia; Fadda, Costantino; Marceddu, Salvatore; Conte, Paola; Del Caro, Alessandra; Piga, Antonio; Università degli Studi di Sassari - Dipartimento di Agraria; Istituto CNR di Scienze delle Produzioni Alimentari - UOS SassariIn Mediterranean countries, and in Sardinia in particular, durum wheat is traditionally used for bread production and modern cultivars have gradually replaced the use of old landraces due to the poor technological quality of the latter. However, recent escalation in customer demand for old varieties thanks to an appreciation of their nutritional properties, as well as ecological issues, has led to the need for technological improvements able to enhance the rheological performance of the old varieties to support their diffusion. The aim of this study was to assess whether the baking performance of an Old Italian wheat cultivar, Russello wheat, could be improved through the addition of different hydrocolloids (methylcellulose, guar, psyllium, xanthan and tara). The effects of two different concentrations (0.5% and 1%) of each hydrocolloid on the rheological properties, pasting, fermentation and microstructural properties of dough were assessed, and the results compared with those obtained using a modern durum wheat cultivar flour. Significant differences were found in the dough obtained with hydrocolloids. In particular, dough extensibility was increased with 1% psyllium or xanthan gum, whereas the gas retention coefficient was increased with all hydrocolloids. The pasting properties were modified via an increase in the final viscosity and setback value compared with control semolina (with the exception of methylcellulose); this resulted in a more homogeneous dough structure with a compact appearance and the absence of any deep interruptions in the gluten network as revealed by scanning electron microscopy.
- ItemHigh-level expression of a recombinant active microbial transglutaminase in Escherichia coli(BioMed Central, 2015-09-15) Salis, Barbara; Spinetti, Gaia; Scaramuzza, Silvia; Bossi, Michele; Saccani Jotti, Gloria; Tonon, Giancarlo; Crobu, Davide; Schrepfer, RodolfoBackground: Bacterial transglutaminases are increasingly required as industrial reagents for in vitro modification of proteins in different fields such as in food processing as well as for enzymatic site-specific covalent conjugation of therapeutic proteins to polyethylene glycol to get derivatives with improved clinical performances. In this work we studied the production in Escherichia coli of a recombinant transglutaminase from Streptomyces mobaraensis (microbial transglutaminase or MTGase) as enzymatically active chimeric forms using different expression systems under the control of both lac promoter or thermoinducible phage lambda promoter. Results: Thermoinducible and constitutive expression vectors were constructed expressing Met-MTGase with chimeric LacZ 1-8 PNP 1–20 or LacZ 1–8 fusion protein under different promoters. After transformed in competent Escherichia coli K12 strains were fermented in batch and fed-bach mode in different mediums in order to select the best conditions of expression. The two most performing fusion protein systems namely short thermoinducible LacZ 1–8 Met-MTGase from NP668/1 and long constitutive LacZ 1-8 PNP 1–20 Met-MTGase from NP650/1 has been chosen to compare both efficiency of expression and biochemical qualities of the product. Proteins were extracted, purified to homogeneity and verified as a single peak obtained in RP-HPLC. The LacZ 1-8 PNP 1–20 Met-MTGase fusion protein purified from NP650/1 exhibited an activity of 15 U/mg compared to 24 U/mg for the shorter fusion protein purified from NP668/1 cell strain. Conclusions: Combining the experimental data on expression levels and specific activities of purified MTGase fusion proteins, the chimeric LacZ 1-8 Met-MTGase, which displays an enzymatic activity comparable to the wild-type enzyme, was selected as a candidate for producing microbial transglutaminase for industrial applications.